• Journal Article

Health assessment of gasoline and fuel oxygenate vapors: Developmental toxicity in mice

Citation

Roberts, L. G., Gray, T. M., Marr, M., Tyl, R., Trimmer, G. W., Hoffman, G. M., ... Schreiner, C. A. (2014). Health assessment of gasoline and fuel oxygenate vapors: Developmental toxicity in mice. Regulatory Toxicology and Pharmacology, 70(2, Suppl. 1), S58-S68. DOI: 10.1016/j.yrtph.2014.06.011

Abstract

CD-1 mice were exposed to baseline gasoline vapor condensate (BGVC) alone or to vapors of gasoline blended with methyl tertiary butyl ether (G/MTBE). Inhalation exposures were 6h/d on GD 5-17 at levels of 0, 2000, 10,000, and 20,000mg/m3. Dams were evaluated for evidence of maternal toxicity, and fetuses were weighed, sexed, and evaluated for external, visceral, and skeletal anomalies. Exposure to 20,000mg/m3 of BGVC produced slight reductions in maternal body weight/gain and decreased fetal body weight. G/MTBE exposure did not produce statistically significant maternal or developmental effects; however, two uncommon ventral wall closure defects occurred: gastroschisis (1 fetus at 10,000mg/m3) and ectopia cordis (1 fetus at 2000mg/m3; 2 fetuses/1 litter at 10,000mg/m3). A second study (G/MTBE-2) evaluated similar exposure levels on GD 5-16 and an additional group exposed to 30,000mg/m3 from GD 5-10. An increased incidence of cleft palate was observed at 30,000mg/m3 G/MTBE. No ectopia cordis occurred in the replicate study, but a single observation of gastroschisis was observed at 30,000mg/m3. The no observed adverse effect levels for maternal/developmental toxicity in the BGVC study were 10,000/2000mg/m3, 20,000/20,000 for the G/MTBE study, and 10,000/20,000 for the G/MTBE-2 study