• Journal Article

Multiple HIV-1 specific IgG3 responses decline during acute HIV-1: implications for detection of incident HIV Infection

Citation

Yates, N. L., Lucas, J. T., Nolen, T., Vandergrift, N. A., Soderberg, K. A., Seaton, K. E., ... Tomaras, G. D. (2011). Multiple HIV-1 specific IgG3 responses decline during acute HIV-1: implications for detection of incident HIV Infection. AIDS, 25(17), 2089-2097. DOI: 10.1097/QAD.0b013e32834b348e

Abstract

OBJECTIVE:: Different HIV-1 antigen specificities appear in sequence post HIV-1 transmission and the IgG subclass response to HIV antigens are distinct from each other. The initial predominant IgG subclass response to HIV-1 infection is comprised of IgG1 and IgG3 antibodies with a noted decline in some IgG3 antibodies during acute HIV-1 infection. Thus, we postulate that multiple antigen specific IgG3 responses may serve as surrogates for the relative time since HIV-1 acquisition. DESIGN:: We determined the magnitude, peak, and half-life of HIV-1 antigen specific IgG1 and IgG3 antibodies in 41 HIV-1 infected subjects followed longitudinally from acute infection during the first appearance of HIV-1 specific antibodies through approximately six months post infection. METHODS:: We used quantitative HIV-1 binding antibody multiplex assays and exponential decay models to calculate concentrations of IgG1 and IgG3 antibodies to eight different HIV-1 proteins including gp140 Env, gp120 Env, gp41 Env, p66 Reverse Transcriptase (RT), p31 Integrase, Tat, Nef, and p55 Gag proteins during acute/recent HIV-1 infection. RESULTS:: Among HIV-1 specific IgG3 responses, antigp41 IgG3 antibodies were the first to appear. We found that antigp41 Env IgG3 and antip66 RT IgG3 antibodies, in addition to anti-Gag IgG3 antibodies, each consistently and measurably declined after acute infection, in contrast to the persistent antigen specific IgG1 responses. CONCLUSIONS:: The detailed measurements of the decline in multiple HIV-specific IgG3 responses simultaneous with persistent IgG1 responses during acute and recent HIV-1 infection could serve as markers for detection of incident HIV infection