Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.
A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1
Duckworth, O. W., Markarian, D. S., Parker, D. L., & Harrington, J. M. (2017). A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1. Journal of Microbiological Methods, 135, 11-13. https://doi.org/10.1016/j.mimet.2017.01.019