• Journal Article

A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1

Citation

Duckworth, O. W., Markarian, D. S., Parker, D. L., & Harrington, J. M. (2017). A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1. Journal of Microbiological Methods, 135, 11-13. DOI: 10.1016/j.mimet.2017.01.019

Abstract

Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.