Synthesis of [3'-3H]Taxol and [13-3H]Taxol
7‐Trietylsilylbaccatin III (11) was coupled with cis‐1‐benzoyl‐3‐triethylsiloxy‐4‐(3′‐bromophenyl)azetidin‐2‐one. Hydrolysis of the silyl groups gave 3″‐bromotaxol which was reduced with tritium gas to give[3″‐3H]taxol with specific activity of 19.3 Ci mmol. Reduction of 7‐triethylsilyl‐13‐oxobaccatin III with [3H]borane‐tetrahydrofuran complex gave 7‐triethylsilyl[13‐3H]baccatin III (17). Coupling of 17 with cis‐3‐triethylsiloxy‐4‐phenyl‐(3R,4S)‐azetidin‐2‐one and hydrolysis gave [13‐3H]taxol with specific activity of 1.66 Ci/mmol.
Taylor, G., Thornton, S., Tallent, C., & Kepler, J. (1993). Synthesis of [3'-3H]Taxol and [13-3H]Taxol. Journal of Labelled Compounds and Radiopharmaceuticals, 33(6), 501-515. https://doi.org/10.1002/jlcr.2580330608