The Preparation of 5'-Iodo-125I-D8-THC; A Radioligand for the Radioimmunoassay of Cannabinoids
Nucleophilic exchange of 5′‐iodo‐Δ8‐THC and sodium iodide‐125 afforded 5′‐iodo‐125I‐Δ8THC with a maximum specific activity of 42 Ci/mmole. The specific activity could be increased to at least 100 Ci/mmole by iodide‐125 displacement of the corresponding 5′‐tosylate. A hapten 5′‐carboxy‐14C‐Δ9‐THC, for use in conjunction with this radioligand, was synthesized from 5′‐bromo‐Δ8‐THC.
Pitt, C., Seltzman, H., Setzer, S. R., & Williams, D. L. (1980). The Preparation of 5'-Iodo-125I-D8-THC; A Radioligand for the Radioimmunoassay of Cannabinoids. Journal of Labelled Compounds and Radiopharmaceuticals, 17(5), 681-689. https://doi.org/10.1002/jlcr.2580170510