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Bioactivity-guided fractionation of the leaves of Ocimum gratissimum L. (Lamiaceae), using the brine shrimp lethality test, led to the isolation of oleanolic acid. Oleanolic acid showed bioactivity against a panel of six human solid tumor cell lines, the nematode Caenorhabditis elegans and yellow fever mosquito larvae Aedes aegypti . Details of the isolation and bioactivities are described.