A method is described for use in analysis of samples from pharmaceutical profiling of early drug discovery compounds. The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. A primary method, which is useful for 80–90% of compounds, and a secondary method, which is useful for a majority of the remaining compounds, are described. No analytical HPLC column is used and the analysis rate is approximately 50 samples/h. Specificity is obtained using MRM analysis. Application of the method for high capacity analysis of metabolic stability samples is described.
Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery
Kerns, EH., Kleintop, T., Little, D., Tobien, T., Mallis, L., Di, L., Hong, Y., & McConnell, OJ. (2004). Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery. Journal of Pharmaceutical and Biomedical Analysis, 34(1), 1-9. https://doi.org/10.1016/j.japna.2003.03.001