Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery
Kerns, E. H., Kleintop, T., Little, D., Tobien, T., Mallis, L., Di, L., ... McConnell, O. J. (2004). Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery. Journal of Pharmaceutical and Biomedical Analysis, 34(1), 1-9. DOI: 10.1016/j.japna.2003.03.001
A method is described for use in analysis of samples from pharmaceutical profiling of early drug discovery compounds. The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. A primary method, which is useful for 80–90% of compounds, and a secondary method, which is useful for a majority of the remaining compounds, are described. No analytical HPLC column is used and the analysis rate is approximately 50 samples/h. Specificity is obtained using MRM analysis. Application of the method for high capacity analysis of metabolic stability samples is described.