• Journal Article

High-molecular-weight polypeptide substrates for phospholysine phosphatases

Citation

Wong, C., Wu, W., Obenshain, S. A., Diah, S. K., Faiola, B., & Kennelly, P. J. (1994). High-molecular-weight polypeptide substrates for phospholysine phosphatases. Analytical Biochemistry, 222(1), 14-18.

Abstract

The detection and characterization of the phosphatases responsible for the dephosphorylation of N-phosphoryl groups, such as phospholysine, in proteins has been frustrated in large measure by a lack of suitable substrates for the assay of these enzymes. Herein we describe the preparation of phospholysine-containing amino acid homo- and heteropolymers by chemical means, and their use in a simple, nonradioactive assay for phospholysine phosphatase activity