• Article

Ethylene dibromide: negative results with the mouse dominant lethal assay and the electrophoretic specific-locus test

Ethylene dibromide (1,2-dibromoethane; EDB) was tested for the induction of dominant lethal and electrophoretically-detectable specific-locus mutations in the germ cells of DBA/2J male mice. Males were treated with a single intraperitoneal injection of 100 mg/kg EDB and mated to two C57BL/6J females. In the dominant lethal assay, matings were carried out to measure the effect of EDB on meiotic and postmeiotic stages; germ cells representing spermatogonial stem cells were analyzed in the electrophoretic specific-locus test. Neither of these germ cell tests produced any evidence that EDB is a germ cell mutagen. It appears from these data and those reported in the literature that EDB, a genotoxic carcinogen that affects male fertility in some mammalian species, is not mutagenic in the germ cells of the male mouse

Citation

Barnett, L., Lovell, D. P., Felton, C., Gibson, B. J., Cobb, R., Sharpe, D. S., ... Lewis, S. (1992). Ethylene dibromide: negative results with the mouse dominant lethal assay and the electrophoretic specific-locus test. Mutation Research, 282(2), 127-133.