S-nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This chapter provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties.
Direct methods for detection of protein S-nitrosylation
Devarie-Baez, N. O., Zhang, D., Li, S., Whorton, A. R., & Xian, M. (2013). Direct methods for detection of protein S-nitrosylation. Methods, 62(2), 171-176. https://doi.org/10.1016/j.ymeth.2013.04.018
Abstract
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