Chemical shift assignments and secondary structure prediction of the phosphorelay protein VanU from Vibrio anguillarum
Bobay, B. G., Thompson, R. J., Milton, D. L., & Cavanagh, J. (2014). Chemical shift assignments and secondary structure prediction of the phosphorelay protein VanU from Vibrio anguillarum. Biomolecular NMR Assignments, 8(1), 177-179. DOI: 10.1007/s12104-013-9478-2
Vibrio anguillarum is a biofilm forming Gram-negative bacterium that survives prolonged periods in seawater and causes vibriosis in marine life. A quorum-sensing signal transduction pathway initiates biofilm formation in response to environmental stresses. The phosphotransferase protein VanU is the focal point of the quorum-sensing pathway and facilitates the regulation between independent phosphorelay systems that activate or repress biofilm formation. Here we report the H-1, C-13, and N-15 backbone and side chain resonance assignments and secondary structure prediction for VanU from V. anguillarum.