• Article

Calbindin D-28K interacts with Ran-binding protein M


Lutz, W., Frank, E. M., Craig, T. A., Thompson, R., Venters, R. A., Kojetin, D. J., ... Kumar, R. (2003). Calbindin D-28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy. Biochemical and Biophysical Research Communications, 303(4), 1186-1192. DOI: 10.1016/S0006-291X(03)00499-6


Calbindin D-28K is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D-28K interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR, timescale. We suggest that the interaction between calbindin D-28K and Ran-binding protein M may be important in calbindin D-28K function. (C) 2003 Elsevier Science (USA). All rights reserved.