Calbindin D-28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy

John Cavanagh; Richele Jo Thompson; W Lutz; EM Frank; TA Craig; Richele Jo Thompson; RA Venters; DJ Kojetin; John Cavanagh; R Kumar

Calbindin D-28K interacts with Ran-binding protein M

identification of interacting domains by NMR spectroscopy

Lutz, W., Frank, EM., Craig, TA., Thompson, R., Venters, RA., Kojetin, DJ., Cavanagh, J., & Kumar, R. (2003). Calbindin D-28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy. Biochemical and Biophysical Research Communications, 303(4), 1186-1192. https://doi.org/10.1016/S0006-291X(03)00499-6

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Abstract

Calbindin D-28K is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D-28K interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR, timescale. We suggest that the interaction between calbindin D-28K and Ran-binding protein M may be important in calbindin D-28K function. (C) 2003 Elsevier Science (USA). All rights reserved.