Calbindin D-28K interacts with Ran-binding protein M identification of interacting domains by NMR spectroscopy
Calbindin D-28K is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D-28K interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR, timescale. We suggest that the interaction between calbindin D-28K and Ran-binding protein M may be important in calbindin D-28K function. (C) 2003 Elsevier Science (USA). All rights reserved.
Lutz, W., Frank, E. M., Craig, T. A., Thompson, R., Venters, R. A., Kojetin, D. J., ... Kumar, R. (2003). Calbindin D-28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy. Biochemical and Biophysical Research Communications, 303(4), 1186-1192. DOI: 10.1016/S0006-291X(03)00499-6