• Chapter

Bone marrow-derived dendritic cells

Citation

Roney, K. (2013). Bone marrow-derived dendritic cells. In I. C. Allen (Ed.), Mouse Models of Innate Immunity: Methods and Protocols. Methods in Molecular Biology series (pp. 71-76). New York, NY: Humana Press / Springer. DOI: 10.1007/978-1-62703-481-4_9

Abstract

While much is understood about dendritic cells and their role in the immune system, the study of these cells is critical to gain a more complete understanding of their function. Dendritic cell isolation from mouse body tissues can be difficult and the number of cells isolated small. This protocol describes the growth of large number of dendritic cells from the culture of mouse bone marrow cells. The dendritic cells grown in culture facilitate experiments that may require large number of dendritic cells without great expense or use of large number of mice