2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomes

Kim, H., Cope, M. B., Herring, R., Robinson, G., Wilson, L., Page, G., & Barnes, S. (2008). 2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomes. Journal of Proteome Research, 7(11), 4638-4650. DOI: 10.1021/pr800121b

Rat mammary gland proteomes at day 21 (prepubertal) and day 50 (late puberty) were compared by 2D difference gel electrophoresis. Two-hundred fifty-one spots were significantly different (p < 0.05) in abundance. Peptide mass fingerprint analysis of a subset of these proteins identified two significantly over-represented classes including structural and blood proteins (increased), and metabolism-relevant proteins (reduced) in day 50 relative to day 21 glands. This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points.