RTI Press Method Report

Quantitative real-time polymerase chain reaction (qPCR) of filamentous fungi in carpet

Black, J. (September 2009).

Detection of fungi contamination in the built environment remains problematic, because fungi inhabit surfaces such as carpet that becomes dirty, which makes genomic DNA extraction difficult, according to a new study by researchers at RTI International.

The study demonstrates that milling spores in lysis buffer and removing cell debris by filtration provides high-molecular-weight DNA, which could then be uses for quick and accurate quantitation of fungi from carpet.

Full Document: RTI Press - Methods Report PDF

Permanent Link: doi:10.3768/rtipress.2009.mr.0011.0909

Full Citation: Black, J. (September 2009). Quantitative real-time polymerase chain reaction (qPCR) of filamentous fungi in carpet. RTI Press Publication No. MR-0011-0909.

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Abstract

Detection of fungi contamination in the built environment remains problematic, because fungi inhabit surfaces such as carpet that becomes dirty, which makes genomic DNA extraction difficult, according to a new study by researchers at RTI International.

The study demonstrates that milling spores in lysis buffer and removing cell debris by filtration provides high-molecular-weight DNA, which could then be uses for quick and accurate quantitation of fungi from carpet.

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