Stable dry powder formulation for nasal delivery of anthrax vaccine
Wang, SH., Kirwan, SM., Abraham, SN., Staats, HF., & Hickey, A. (2012). Stable dry powder formulation for nasal delivery of anthrax vaccine. Journal of Pharmaceutical Sciences, 101(1), 31-47. https://doi.org/10.1002/jps.22742
Abstract
There is a current biodefense interest in protection against anthrax. Here, we developed a new generation of stable and effective anthrax vaccine. We studied the immune response elicited by recombinant protective antigen (rPA) delivered intranasally with a novel mucosal adjuvant, a mast cell activator compound 48/80 (C48/80). The vaccine formulation was prepared in a powder form by sprayfreeze-drying (SFD) under optimized conditions to produce particles with a target size of D50 = 25?mu m, suitable for delivery to the rabbit nasal cavity. Physicochemical properties of the powder vaccines were characterized to assess their delivery and storage potential. Structural stability of rPA was confirmed by circular dichroism and attenuated total reflectanceFourier transform infrared spectroscopy, whereas functional stability of rPA and C48/80 was monitored by cell-based assays. Animal study was performed using a unit-dose powder device for direct nasal application. Results showed that C48/80 provided effective mucosal adjuvant activity in rabbits. Freshly prepared SFD powder vaccine formulations or powders stored for over 2?years at room temperature elicited significantly elevated serum PA-specific and lethal toxin neutralization antibody titers that were comparable to that induced by intramuscular immunization with rPA. Nasal delivery of this vaccine formulation may be a viable alternative to the currently licensed vaccine or an attractive vaccine platform for other mucosally transmitted diseases. (c) 2011 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 101:3147, 2012
To contact an RTI author, request a report, or for additional information about publications by our experts, send us your request.