Strengths and limitations of commercial tests for hepatitis C virus RNA quantification
The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay
Morishima, C., Chung, M., Ng, KW., Brambilla, D., & Gretch, DR. (2004). Strengths and limitations of commercial tests for hepatitis C virus RNA quantification. Journal of Clinical Microbiology, 42(1), 421-425.