Preparation of high specific activity tritium labeled 6 alpha,9,-difluoro-11 beta,21-dihydroxy-16 alpha,17-[(1-methylethylidene)bis(oxy)]pregna-1,4-diene-3,20-one, fluocinolone acetonide
Fluocinolone acetonide was tritiated by selective reduction of the 1,2-double bond of the O-protected analog under tritium, followed by re-establishment of the 1,2-double bond and deprotection. Protection of both hydroxyl functionalities was required. The product was obtained with specific activity 36.8?Ci/mmol. Copyright © 2009 John Wiley & Sons, Ltd.