Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain
A novel off-resonance rotating-frame N-15 NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 mu s in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the beta-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the beta-sheets. Flexibility of the loop containing the Arg-Gly-Asp (RGD) tripeptide may affect the accessibility of this motif in protein-protein interactions.
Akke, M., Liu, J., Cavanagh, J., Erickson, HP., & Palmer III, AG. (1998). Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain. Nature Structural and Molecular Biology, 5(1), 55-59.