Two new protocols for the three-dimensional, triple resonance, constant-time HCA(CO)N NMR experiment are presented that significantly increase the experimental resolution attainable in the C(alpha) frequency dimension. Experimental verification of the new experiments is provided by spectra of the IIA domain of glucose permease from Bacillus subtilis.
IMPROVED RESOLUTION IN 3-DIMENSIONAL CONSTANT-TIME TRIPLE RESONANCE NMR-SPECTROSCOPY OF PROTEINS
Palmer III, AG., Fairbrother, WJ., CAVANAGH, J., WRIGHT, PE., & Rance, M. (1992). IMPROVED RESOLUTION IN 3-DIMENSIONAL CONSTANT-TIME TRIPLE RESONANCE NMR-SPECTROSCOPY OF PROTEINS. Journal of Biomolecular NMR, 2(1), 103-108.
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