• Article

Identification of communication networks in SpoOF


Feher, V. A., Tzeng, Y. L., Hoch, J. A., & Cavanagh, J. (1998). Identification of communication networks in SpoOF: a model for phosphorylation-induced conformational change and implications for activation of multiple domain bacterial response regulators. FEBS Letters, 425(1), 1-6.


Fundamental to understanding the mechanism by which phosphorylation activates bacterial signal transduction response regulator proteins is the identification of regions and residues that are responsible for the phosphorylation-induced conformational change, Here we review results from structural and protein dynamics investigations, and combine them with mutagenesis studies on the response regulator protein SpoOF to suggest a model in which a network of buried and surface residues link surface regions required for protein:protein interactions to the site of phosphorylation. The network described for SpoOF may provide pathways through which information is transmitted from the site of phosphorylation, propagating a conformational change many angstroms away, The general applicability of the communication network model for all bacterial response regulator proteins is discussed. (C) 1998 Federation of European Biochemical Societies.