Gas chromatographic-electron-impact mass fragmentometric determination of lysergic acid diethylamide in urine
A sensitive method for the detection and quantitation of lysergic acid diethylamide (LSD) in urine was developed. After initial solvent extraction, the compound was further purified by liquid-liquid extraction or by solid-phase extraction. The trimethylsilyl derivative of LSD was detected by gas chromatography-mass spectrometry (GC-MS) operated in the electron-impact mode with selected-ion monitoring. The presence of LSD was confirmed by comparing retention times and relative abundances of ions of unknowns with that of a standard. The recovery of this procedure was> 89%. The intra-run and inter-run coefficients of variation were 5% and 7%, respectively. This procedure allows detection of LSD concentrations as low as 29 pg/ml. Quantitation of LSD was linear over the concentration range 50–2000 pg/ml.
Paul, B. D., Mitchell, J., Burbage, R., Moy, M., & Sroka, R. (1990). Gas chromatographic-electron-impact mass fragmentometric determination of lysergic acid diethylamide in urine. Journal of Chromatography, 529, 103-112. DOI: 10.1016/S0378-4347(00)83811-6