Protein S-sulfhydration (forming -S-SH adducts from cysteine residues) is a newly defined oxidative posttranslational modification and plays an important role in H2S-mediated signaling pathways. In this study we report the first selective, “tag-switch” method which can directly label protein S-sulfhydrated residues by forming stable thioether conjugates. Furthermore we demonstrate that H2S alone cannot lead to S-sulfhydration and that the two possible physiological mechanisms include reaction with protein sulfenic acids (P-SOH) or the involvement of metal centers which would facilitate the oxidation of H2S to HSC.
Detection of protein S-sulfhydration via a tag-switch technique
Zhang, D., Macinkovic, I., Devarie-Baez, N. O., Pan, J., Park, C-M., Carroll, K. S., Filpovic, M. R., & Xian, M. (2013). Detection of protein S-sulfhydration via a tag-switch technique. Angewandte Chemie - International Edition, 53(2), 575–581. https://doi.org/10.1002/anie.201305876
Abstract
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