BIBAC technology: progress and prospects
A BIBAC vector serves dual functions as a vector for the construction of large insert genomic DNA libraries and as a binary vector for Agrobacterium- mediated plant transformation. Because BIBAC technology narrows the temporal gap between the identification of candidate genomic library clones and plant transformation to complement a phenotype, this technology should greatly facilitate the identification of agriculturally important genes that are known only by their phenotype. The BIBAC name and its functions are a hybrid of the widely used binary (e. g. pBI and pBIN) vectors for plant transformation and the bacterial artificial chromosome (BAC) vectors for the construction of large insert DNA libraries. With methods for the identification of closely linked flanking markers in hand, “chromosome landing” is the new ideal for identification of candidate genomic library clones. If the genomic library is constructed in a BIBAC vector, it is now possible to “land and leap” directly into plant transformation. The step from clone identification to plant transformation experiments simply requires a Escherichia coli mini- plasmid prep and transformation of a strain of A. tumefaciens suitable for transformation of the plant system of interest.