Mass Spectrometry

Mass Spectrometry Technician

Mass spectrometry (MS) is a powerful analytical technique used to identify unknown compounds, quantify known materials, and determine the structural and chemical properties of molecules. RTI uses MS analysis for basic and applied research within multiple disciplines, including proteomics, drug discovery and development, environmental contaminants, and inorganic (trace metals) chemistry.

Molecular Epidemiology, Genomics, Environment, and Health Laboratory

Applied Biosystems Proteomics 4700 Tandem MALDI TOF/TOF Mass Spectrometer

A high-performance MALDI mass spectrometer with true tandem capabilities. Equipped with 200 Hz Nd:Yag laser, ultimate mass resolution to 30,000 at m/z 12,000 Da, sensitivity quoted at 10 amol of a single peptide analyte. Can accept up to three collision gases, integrated Oracle database for storage of results, capable of fully automated operation; data-dependent MS/MS analyses can be performed. Analysis is automated using a plate-loader with a capacity of up to 24 plates. The autoloader has a built-in bar code reader to allow rapid plate-to-plate transfer. This integrated system runs on the GPS Explorer software platform for data management.

LECO Pegasus® 4D GC x GC TOF Mass Spectrometer

GCxGC, combined with the Pegasus HT TOFMS, changes the dynamic when analyzing complex samples. No longer limited to two analytical dimensions, the Pegasus 4D GCxGC-TOFMS system provides four dimensions of analytical resolution for a more complete sample analysis. GCxGC enables the detection of hundreds to thousands more compounds than previously seen using conventional one-dimensional GC. Unique processing capabilities, such as sample comparisons, peak alignment, and scripting functionality, allow for automated mining of complex GCxGC data to extract previously unidentifiable similarities and differences.

Thermo Electron LTQ Linear Ion Trap Mass Spectrometer

A linear ion trap mass spectrometer capable of advanced scanning modes, including data dependent scans, ion mapping, neutral ion mapping, parent ion mapping, user-selectable dynamic exclusion, Nth-order triple play, data-dependent zoom map, data-dependent ion tree and total ion map, custom data dependence, real-time modifications, and Nth-order neutral loss data dependence. Equipped with conventional ESI probe with orthogonal spray. New Objective Picoview nanospray source is also available for static and dynamic (LC) nanospray experiments. Mass range to 20,000 Da, normal operating ranges at 2,000 and 4,000 Da. Integrated Xcalibur data system allows rapid programming of data-dependent LC-MS/MS experiments. Control of LC is available from Xcalibur using a software plug-in.

Thermo Electron LTQ ETD Linear Ion Trap Mass Spectrometer

A linear ion trap mass spectrometer capable of ion-ion reactions for electron transfer or proton transfer. This system is equipped with a second (negative ion chemical ionization) ion source to generate counter ions for reaction with oppositely charged ions generated by electrospray. The system is controlled and programmed using ion trap control language (ITCL). It is used for instrument development and fundamental research in proteomics.

Thermo Electron LCQ-Deca-XP Ion Trap Mass Spectrometer

A high-performance quadrupole ion trap mass spectrometer for electrospray MS analyses. Equipped with conventional ESI probe with orthogonal spray. New Objective Picoview nanospray source is also available for static and dynamic (LC) nanospray experiments. Mass range to 20,000 Da, normal operating ranges at 2,000 and 4,000 Da. Integrated Xcalibur data system allows rapid programming of data-dependent LC-MS/MS experiments. Control of LC is available from Xcalibur using a software plug-in.

Drug Discovery and Development Laboratory

Agilent 7890 GC with 7000B MS/MS System

The 7000B Triple Quadrupole GC/MS/MS is equipped with an exchangeable Ei and CI (positive and negative) ion source and a Combipal autosampler that allows for liquid, headspace and SPME-headspace injection. The instrument comes with Agilent MassHunter software for data acquisition and qualitative/quantitative analysis. The instrument can perform MRM, product-ion, precursor-ion and neutral loss scans.

Agilent 1200 Rapid Resolution LC with Applied Biosystems API-5000 Mass Spectrometer

Agilent 1200 Series solvent degasser, binary pump, thermostatted 96 well plate autosampler, thermostatted column compartment and diode array detector. API 5000 triple quadrupole mass spectrometer with turbo ion spray, APCI, and photospray interfaces.

Agilent 1100 HPLC with Applied Biosystems API-4000 Mass Spectrometer

Agilent 1100 Series solvent degasser, binary pump, thermostatted 96 well plate autosampler, thermostatted column compartment and diode array detector. API 4000 triple quadrupole mass spectrometer with turbo ion spray, APCI, and photospray interfaces.

Agilent 1100 HPLC with Applied Biosystems API-4000 Mass Spectrometer

Agilent 1100 Series solvent degasser, binary pump, thermostatted 96 well plate autosampler, Eppendorf column heater, and dual wavelength UV detector. API 4000 triple quadrupole mass spectrometer with turbo ion spray, APCI, and photospray interfaces.

Agilent 1100 HPLC with Applied Biosystems API-3000 Mass Spectrometer

Agilent 1100 Series solvent degasser, quaternary pump, thermostatted 96 well plate autosampler, thermostatted column compartment and diode array UV detector. API 3000 triple quadrupole mass spectrometer with turbo ion spray and APCI interfaces.

Perkin Elmer 200-micro HPLC with Agilent 1100 Series UV Detector and Applied Biosystems API-150EX Mass Spectrometer

Perkin Elmer 200 Micro Series autosampler and binary pump system, plus online degasser. Agilent 1100 Series dual wavelength UV detector. API 150EX single quadrupole mass spectrometer with turbo ion spray and APCI interfaces.

Capillary GC/MS System

Agilent 5890 gas chromatograph equipped with a 7673A automatic liquid sampler and split/splitless injection ports. Agilent 5989A MS Engine equipped with diffusion pumps and isolation valves, electron impact ionization source, chemical ionization source (positive and negative ion). Heated direct insertion probe and thermospray ionization probe. Chemstation datastation with searchable Wiley and NIST mass spectral databases.

Environmental Sciences Laboratory

GC/MS – Agilent 6890/5973

This system is configured both for solvent injection and thermal desorption using a Perkin Elmer turbomatrix automated thermal desorption unit (ATD).

Trace Metals Analysis Laboratory

ICP-MS; Thermo Electron Corporation; X Series and ICP-MS; Thermo Electron Corporation; X Series II

The Thermo X-Series ICP-MS instruments are designed to provide rapid multi-element analysis in a variety of challenging matrices to sub-parts per billion (ppb) levels. The lens stack design coupled with collision cell technology provides the chemist with a powerful tool to overcome polyatomic interferences that can severely degrade sensitivity and specificity on a standard quadrupole ICP-MS. With multiple interface choices, an adjustable temperature controlled spray chamber, and multiple collision cell gas choices, the X-Series can be configured to solve almost any analytical challenge.

Sector Field (SF)-ICP-MS; Thermo Element 2

SF-ICP-MS can eliminate almost all polyatomic interferences and offers the maximum sensitivity for trace metals analysis. Detection limits in the low ppq to sub-ppb levels are achievable for most elements. The SF-ICP-MS couples with HPLC/UPLC systems to provide a powerful tool for speciation of metals such as arsenic, vanadium, tin, and chromium.

General Applications Laboratory

Agilent 5973 GC Mass Spectrometer

Unit resolution quadrupole; electron impact (eI) operating mode; capillary GC with autosampler; external direct insertion probe (DIP), Chem Station software with searchable NIST spectral library.

PE-Sciex API-365 Triple Quadrupole Mass Spectrometer

A triple quadrupole mass spectrometer capable of MRM, product-ion, precursor-ion, and neutral loss scans. Mass range of 10 to 3000 Da. Equipped with conventional electrospray and atmospheric pressure chemical ionization interfaces. Analyst 1.1 software controls mass spectrometer and LC system.