Proteomics Research

Proteomics Expertise

Proteomics research at RTI combines advanced mass spectrometry and proteomics with the organizational reliability and stability necessary to deliver tangible solutions. Our distinguished staff has a combined 30+ years of experience in the field of proteomics. We also have a strong record of publishing the results of our work in the scientific literature.

In recent years, we have focused on developing alternative methods to separate complex peptide mixtures as well as informatics tools such as advanced mass spectrometry to better identify proteins. We developed a platform to separate complex peptides based on their isoelectric point, using commercially available tools. We use the information obtained from this type of separation to further enable the discovery of novel gene open-reading frames by mass spectrometry and the detection of gene-coding single nucleotide polymorphisms.

Proteomics Projects

These technology developments are being practiced in a major cross-RTI program funded by the National Institutes of Health. The program goal is to understand the mechanisms of innate and adaptive immunity to typhoid and cholera. Building on our experience in instrumentation development and microbial proteomics, we are engaged in a project to develop next-generation tools to analyze biothreat agents using protein mass spectrometry.

Examples of current research and capabilities include the following projects:

  • Mass spectrometry technology development for biothreat agent detection
  • Explosives analysis by mass spectrometry
  • Quantitative analysis of bioactive peptides in biological matrices
  • Structural confirmation/purity assessment of synthetic peptide analogues
  • Analysis/sequence confirmation of recombinant proteins
  • Accurate mass measurement on small molecules by MALDI TOF and ESI-MS
  • Proteome profiling of hyperthermophilic bacteria
  • Determination of protein turnover profiles by mass spectrometry

Proteomics Tools and Facilities

To conduct this research, our scientists use a suite of cutting-edge proteomics and mass spectrometry tools, including the following capabilities:

  • Quantitative 2-D gel electrophoresis platform, featuring differential in-gel electrophoresis technology (DIGE) and automated spot picker
  • Automated sample preparation platform for shotgun IPG-IEF
  • Two linear ion trap mass spectrometers, both equipped with nanospray ionization, one with electron transfer dissociation
  • Three-dimensional ion trap mass spectrometer equipped with nanospray ionization
  • Triple-quadruple mass spectrometer equipped with electrospray ionization and atmospheric pressure chemical ionization for quantitative analysis
  • High-performance MALDI TOF-TOF, with LC-MALDI capability available
  • Conventional flow and nanoflow HPLC platforms